Lineage-specific genetic ablation
Genetic ablation occurs when a gene is deemed “null” through the homologous genetic recombination of a gene. It is utilized in the selective suppression of a specific cell line or cell type. This genetic engineering technique does not limit growth suppression to just the activity of an individual gene. Specific cell … Se mer In 1990, the gene knockout technique was just developing. There was a lack of information on the initial events that occur throughout the development of the vertebrate embryo. In order to form a better understanding, the … Se mer Temporal control of gene expression and ablation can be attributed to evolving transgenic and gene-therapy technologies. These technologies are enhanced by an understanding of the … Se mer The ability to selectively remove cells by ablation is monumental in the study of the development of eukaryotic biology, contributing greatly to the study of the origin, fate, or function of the cells. Genetic ablation occurs through the delivery of a toxin or death … Se mer Nettet31. jul. 1987 · Cell lineage ablation in transgenic mice by cell-specific expression of a toxin gene. A method of deleting specific cell lineages has been developed that …
Lineage-specific genetic ablation
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Nettetthe genetic program is thought to account for the estab- lishment of cell lineages in multicellular organisms. Once different lineages are established, the cells of these line- … Nettet7 timer siden · Sex-specific gene expression of sterile giant danio by RT-qPCR Total RNA was extracted using TriReagent extraction and LiCl precipitation according to the manufacturer’s instructions.
Nettet15. sep. 1998 · Previously, methods to selectively kill cells by genetic means have been used primarily for studies of lineage specification during development (19–23), to some extent reflecting the constraints of … Cell ablation (from Latin Cellula "small chamber" and Ablatio "removal"). Also known as tissue ablation, cell ablation is a biotechnological tool for studying cell lineage. The process consists of selectively destroying or removing cells in an organism. For example, a laser beam or controllable gene promoter for a toxin gene can be used to destroy a selected amount of cells.
Nettet10. apr. 2024 · Previously, we have shown that either disruption of the Hoxb8 gene or ablation of a microglial subpopulation, Hoxb8 microglia, results in mice exhibiting both chronic anxiety and OCSD-like ... Nettet11. des. 1987 · Lineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a …
Nettet26. apr. 2024 · Current research on cell fate mapping of endogenous CSCs largely relies on the conventional genetic lineage tracing technology that utilizes the promoters of specific markers expressed by CSCs. However, some proposed stem cell markers are nonspecific to putative CSCs, 12 , 19 – 21 as they are also expressed by cardiomyocytes.
Nettet15. mar. 2024 · In this present study, we performed bulk RNA-sequencing (RNA-seq) in hESC-derived articular and growth plate cartilage to identify lineage-specific gene expression, and compared them to developing fetal cartilage. We also performed ATAC-sequencing (ATAC-seq) to define the accompanying regulatory landscapes in hESC … bulletin graphicsNettetTo enable more specific cell lineage tracing and ablation through dual recombinase activity, we generated two mouse lines that render Dre- or Dre+Cre-mediated … bulletin headersNettet1. nov. 2024 · Cell-specific expression of genes offers the possibility to use their promoters to drive expression of Cre-recombinase, thereby allowing for detailed … bullet in head icd 10Nettet20. mai 2005 · A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin (DT). bulleting text in excelNettetGene Deletion. Cell-specific gene deletion and genetic epistasis experiments has established that in vivo osteocalcin is the ligand of Gprc6a that explains the regulation … bullet in head cartoonNettet5. aug. 2013 · Main Text. Recent advances in cellular reprogramming have re-awakened interest in lineage-specification mechanisms and challenged views about the very nature of commitment itself [1].From a molecular viewpoint, lineage identity results from the accumulation of stable lineage-specific transcription factor complexes at the … bulleting shortcutNettet14. apr. 2024 · Examination of lineage-specific markers identified clusters with gene signatures of osteoblast (cluster 4), osteocyte (cluster 5), adipocyte (cluster 7), and chondrocyte (cluster 8 and 9, Figure 1C). Chondrocyte clusters, while unexpected, likely represent growth plate chondrocytes released from bone fragments during enzymatic … bulletinhealthcare.com